Preparation of silver nanoparticles by extract of Yinqiao Jiedu Mixture waste and its antioxidative and antibacterial activity evaluation / 中草药

Objective: To develop a novel method to synthesize silver nanoparticles (AgNPs) by using the extract of Yinqiao Jiedu Mixture waste, evaluate the effects of biosynthesis parameters on the formation and polydispersity of AgNPs, and investigate the antioxidative and antibacterial activity. Methods: The formation of AgNPs was confirmed by UV-visible spectroscopy; The size, polydispersity, surface and morphology features of AgNPs were characterized by laser granularity analyzer and transmission electron microscopy; The antioxidative and antibacterial activities of AgNPs were evaluated by calculating the scavenging rate for DPPH and A600 for both Escherichia coli and Staphylococcus aureus, respectively. Results: By using Yinqiao Jiedu Mixture waste, the AgNPs could be prepared at ambient temperature. The size and polydispersity index of the synthesized AgNPs were sensitive to the biosynthesis parameters, such as pH of extract and material proportion with the average size distribution was 14.2-94.8 nm, offering a size-controlled synthetic method for AgNPs. And when the pH was 6.0, the polydispersity could reach the best. The AgNPs could be obtained with high yield and small size at pH 10.0, material proportion 3:1 after reacting 2 h, which were quasi-spherical in shape with average size of (24.0 ± 0.3) nm and covered by anion [Zeta potential: (-23.1 ± 0.2) mV]. The synthesized AgNPs also revealed significant inhibition activities for the growth of E. coli and S. aureus with MIC 50.0 and 25.0 μg/mL, respectively, and potent antioxidative activity with scavenging rate for DPPH 71.1% when adding 100 μg/mL of AgNPs. Conclusion: The extract of Yinqiao Jiedu Mixture waste can be used to synthesize AgNPs with small size at ambient temperature; The biosynthesis parameters have significant effects on the average size and polydispersity index of AgNPs; The synthesized AgNPs have potent antioxidative and antibacterial activity.

Advances in pharmacological effects of higenamine on cardiovascular system / 药学学报

Higenamine (HG) is an active cardiotonic component isolated from Aconite. Chinese and foreign scholars have done a lot of research on the metabolism and pharmacological effects of HG, which confirmed that it has cardiovascular pharmacological effects of cardiactonic action and vasodilation for the treatment of heart failure and bradycardia, anti-oxidative and anti-apoptotic effects which can be used to protect the heart and reduce heart ischemia and reperfusion injury. In addition, HG inhibits the expression of iNOS mRNA by inhibiting the activity of the transcription factor NF-κB, inhibits the lipopolysaccharide (LPS)-induced NO product, and inhibits platelet aggregation and thrombus formation, thereby improving the experimental septic shock in animals. This article reviews the recent progress in cardiovasular pharmacology of HG, which will contribute to the further development and clinical application of it in the future.

Study on chemical constituents from fruiting bodies of Ganoderma australe / 中国中药杂志

Chemical constituents were isolated from the fruiting bodies of Ganoderma australe by various column chromatographic techniques and HPLC method, and their chemical structures were identified through the combined analysis of physicochemical properties and spectral data. Meanwhile, their α-glucosidase inhibitory activity and anti-oxidative ability were evaluated. Seven compounds were isolated from G. australe and were identified as 6-methoxyl-cyclo-(Phe-Ile)(1), applanoxidic acid A methyl ester(2), ergosta-7,22 E-dien-3β-ol(3), cinnamic acid(4), 5α,8α-epidioxy-(20S,22E,24R)-ergosta-6,22-diene-3β-ol(5), 1-(3, 4-dihydroxyphenyl) ethanone(6), salicylic acid(7) and benzoic acid(8). Among the compounds, compound 1 was a new cyclic dipeptide. Compound 2 was a new lanosta natural product, and compounds 4, 6, 7 and 8 were obtained from G. australe for the first time. Moreover, compounds 4 and 8 exhibited α-glucosidase inhibitory activity with inhibition rates of 36.8% and 34.7%, and compounds 4, 7 and 8 had a certain activity in DPPH free radical scavenging activity with IC_(50) values of 0.168, 0.458 and 0.170 g·L~(-1), respectively. The DPPH radical scavenging rate of compound 1 was 41.1%.

Effects of fungi fraction on growth and anti-oxidative activity of Eleutherococcus senticosus / 中国中药杂志

The present study was conducted to explore the effect of endophytic fungi fraction on growth and anti-oxidative activity of Eleutherococcus senticosus. The growth,yield,contents of MDA,and antioxidant activities were assessed in E. senticosus under five fungi fractions,namely BZ,MH,DT,JS,and XFZ. The results showed that fungi fractions and component significantly affected the growth,low concentration of DT fungi fraction significantly increased the biomass of E. senticosus,reduced the MDA content in cells,and the antioxidant activities of the aqueous extracts were superior to the others. The results indicated that low concentration of DT fungi fraction was the optimum fraction to achieve high yield and quality of E. senticosus.

Phenolic constituents of Pumbago zeylanica and their anti-oxidative effects / 中草药

Objective: To investigate the phenolic compounds from Plumbago zeylanica and their anti-oxidant activities. Methods :The phenolic compounds and their analogues were isolated and purified by silica gel column chromatography, semi-preparative HPLC, and HPLC. Their structures were identified by spectroscopy data. The isolated compounds were tested for anti-oxidant activity by ABTS assay. Results: Twelve phenolic compounds were obtained and identified as (±)-5,5’-dimethoxyl ariciresionl 4’-O-β-D-glucopyranoside (1), tracheloside (2), quercetin-3-O-β-D-galactoside (3), quercetin-3-O-β-D-glucopyranoside (4), quercetin (5), quercetin 3-O-α-L-rhamnoside (6), kaempferol-3-O-α-L-rhamnoside (7), apigenin-6-C-β-D-glucopyranoside (8), luteolin 6-C-β- glucopyranoside (9), polybotrin (10), 3-O-(β-D-glucopyranosyl)-1-(3’,5’-dimethoxy-4’-hydroxyphenyl)-1-propanone (11), and (2E,4E,1’R,3’S,5’R,8’S)-dihydrophaseic acid-3’-O-β-D-glucopyranoside (12). The anti-oxidant activity experiment showed that compounds 3-9 displayed significant anti-oxidative activities. Conclusion: All of the twelve phenolic compounds are isolated from this plant for the first time. The isolated flavones 3-9 displayed significant anti-oxidative activities.

Protective effect of trans-anethole on PC12 cell injury induced by sodium azide / 中国药理学通报

Aim: To investigate the protective effect of trans-anethole on the PC12 cell injury induced by sodium azide. Methods: PC12 cells were exposed to sodium azide with concentration ranged from 9 ∼ 54 mmol · L-1. The survival rate of the cells was determined by CCK-8 assay. The cell morphology was detected with the inverted microscope; the apoptosis of cells was detected by Hoechst 33258 staining; the content of LDH, MDA levels and antioxidant enzyme activities of SOD were determined using the assay kits according to the manufacturer's protocol. Results PC12 cell injury induced by sodium azide presented a dose-effect relationship. The PC12 cell viability was not changed when treated by irans-anethole for 48 h. Pretreatment of PC12 cells with 10 μmol · L-1 and 60 μmol · L∼ trans-anethole for 8 h could obviously reduce cell injury, reduce LDH leakage and MDA level, as well as increase the antioxidant enzyme activities of SOD induced by sodium azide. Conclusions: Trans-anethole exerts significant protective effect on PC12 cell injury induced by sodium azide. The protective mechanism might be executed by increasing the activities of antioxidant enzymes.

Monoside antagonizes triptolide-induced hepatocyte apoptosis the anti-oxidative stress pathway / 南方医科大学学报

<p><b>OBJECTIVE</b>To investigate the protective effect of monoside against triptolide-induced liver injury and explore its molecular mechanism.</p><p><b>METHODS</b>BALB/C mice treated with gastric lavage with triptolide and monoside, either alone or in combination, were examined for changes of hepatic biochemical parameters using the serological method. The growth inhibition rate of HepG2 cells treated with triptolide or monoside or both was assessed with MTT assay, and the cell morphological changes were observed using laser confocal microscopy; the expressions of the target proteins in the antioxidative stress pathway were detected using flow cytometry and Western blotting.</p><p><b>RESULTS</b>In BALB/C mice, gastric lavage of triptolide induced obvious hepatic damage. In HepG2 cells, treatment with triptolide significantly inhibited the cell growth, resulting in a cell viability as low as 72.83% at 24 h; triptolide also induced obvious cell apoptosis and cell nucleus deformation, causing an apoptosis rate of 43.1% in the cells at 24 h. Triptolide significantly reduced the expressions of Nrf2 and HO-1 proteins related with the oxidative stress pathway. Combined treatment with morroniside obviously reversed these changes, resulting in significantly decreased hepatic biochemical parameters and the liver index in BALB/C mice and in significantly lowered cell apoptosis rate, improved cell morphology, and increased Nrf2 and HO-1 protein expressions in HepG2 cells.</p><p><b>CONCLUSIONS</b>Monoside protects against triptolide-induced liver injury possibly by relieving oxidative stress.</p>

Protective roles of Vigna subterranea (Bambara nut) in rats with aspirin-induced gastric mucosal injury / 中西医结合学报

<p><b>OBJECTIVE</b>Vigna subterranea is widely consumed as a traditional staple food in Nigeria and some West African countries. The ethanolic seed extract of V. subterranea (EEVS) was investigated for its gastroprotective effects on aspirin plus pylorus ligation-induced gastric ulcerated rats using an in vivo assay.</p><p><b>METHODS</b>Gastric mucosal ulceration was induced experimentally in Groups 2 to 5 using aspirin plus pylorus ligation. Rats in Group 1 were orally pretreated with 3% Tween 80 only as normal control. Groups 2 to 5 were pretreated with 3% Tween 80 (ulcer group), 20 mg/kg of omeprazole (positive group), and 200 and 400 mg/kg of EEVS (experimental groups), respectively, once daily for 21 days before ulcer induction. Parameters including those for gastric secretions, ulcerated areas and gastric wall histology were assessed. Levels of superoxide dismutase (SOD), glutathione peroxidase (GP), and malondialdehyde (MDA) in the gastric tissue homogenate were also determined.</p><p><b>RESULTS</b>Pretreatment with EEVS significantly (P < 0.05) reduced the ulcer index, gastric volume and total acidity in rats with aspirin plus pylorus ligation-induced ulcer. The pH and mucus of gastric content increased significantly (P < 0.05) while the levels of SOD and GP were observed to be elevated with a reduced amount of MDA. Significant severe gastric mucosal injury was exhibited in the ulcer group and EEVS or omeprazole offered significant (P < 0.05) protection against mucosal ulceration. Histologically, the gastric submucosal layer showed remarkable decrease in edema and leucocytes infiltration compared with ulcer group.</p><p><b>CONCLUSION</b>The study suggests that EEVS offered a protective action against aspirin plus pylorus ligation-induced gastric ulcers in Wistar rats. The protective effect might be mediated via antisecretory, cytoprotective and antioxidative mechanisms.</p>

Strategies and Research Progress of Chinese Medicine in Prevention and Treatment of Diabetic Peripheral Neuropathy / 中国结合医学杂志

Diabetic peripheral neuropathy (DPN) seriously affects the quality of life in patients with type 2 diabetes mellitus. This paper reviews the role of Chinese medicine in the main treatment goal of DPN, including protecting pancreatic β-cells, in the use of antioxidation therapy to delay disease progression, and in the endpoint of neural repair and regeneration. We propose that protecting the body from injury caused by high glucose and oxidative stress, and promoting repair and regeneration of nerves should be the research direction for the prevention and treatment of DPN.

Anti-atherosclerotic effect of extract of traditional Chinese medicine formula Dan-yi-Iian in ApoE(-/-)mice / 中国药理学与毒理学杂志

OBJECTIVE To explore the anti-atherosclerotic effect of the extract of traditional Chinese medicine formula Dan-yi-lian(DYL)and the related mechanism.METHODS Atherosclerosis(AS)mod-el was established in ApoE(-/-)mice with a western diet. The mice were orally administered with differ-ent doses of DYL or vehicle daily for 28 d.The anti-atherosclerotic effect was evaluated by measuring the aortic atherosclerotic lesion area and media thickness with ultrasound imaging and histological sec-tions staining method. The effect on blood lipid was investigated by determining TC, TG, LDL, HDL, Apo-A1, Apo-B, etc. The anti-oxidative activity as assessed by determining the level of SOD, CAT, GSH,GSH-Px and MDA.Western blot analysis was used to determine the effect on ICAM-1,VCAM-1, MMP-2 and TNF-α. RESULTS In Dan-yi-lian administered ApoE(-/-)mice,the plaque area and media thickness were significantly reduced. Meanwhile, serum TC, TG, LDL and Apo-B were decreased, in contrast to the increased level of HDL and Apo-A1.On the other hand,SOD,CAT,GSH and GSH-Px were increased, while MDA was reduced in liver homogenate. In addition, the expression of ICAM-1, VCAM-1,MMP-2 and TNF-α was obviously inhibited by Dan-yi-lian.CONCLUSION Dan-yi-lian exhibit-ed potent anti-athero-sclerotic efficacy,in which the lipid-regulating,anti-oxidative and anti-inflammato-ry mechanism might be involved.

Research progress on flavonoids in plants of Polygonatum Mill. and their pharmacological activities / 中草药

Flavonoid is one of the important natural compounds in Polygonatum genus. A total of 54 compounds of six different structural subtypes have been isolated from eight species of Polygonatum genus. Recent studies have shown that the flavonoid compounds of Polygonatum genus have various bioactivities, including hypoglycemic, anti-inflammatory, anti-oxidative, and antitumor. This study reviewed different structural categories of flavonoids from Polygonatum genus and their pharmacological effects. This work will provide a scientific reference for the further research on flavonoids in Polygonatum genus.

Effects of Hippo signaling on anti-oxidative stress of mesenchymal stem cells in vitro / 中华危重病急救医学

Objective To explore the effects of Hippo signaling on anti-oxidative stress of mouse marrow mesenchymal stem cells (mMSCs) in vitro. Methods mMSCs derived from C57BL/6 mice were identified using fluorescence-activated cell sorting analysis and the capabilities of osteogenic, chondrogenic and adipogenic differentiation were evaluated. 2-deoxy-D-glucose (2-DG) or XMU-MP-1 was used to modulate Hippo signaling. Oxidative stress was induced by H2O2treatment and the effect of oxidative stress induced by H2O2on survival of mMSCs was evaluated using methyl thiazolyl tetrazolium (MTT) assay. The effect of oxidative stress induced by H2O2on Hippo signaling and the effect of Hippo signaling on capability of anti-oxidative stress of mMSCs were analyzed through apoptosis-regulated proteins (Bcl-2 and Bax) using Western Blot. Results Hippo signaling was activated by 2-DG in a concentration-dependent manner and the effect was most prominent by 5 mmol/L of 2-DG [compared with the blank control group, large tumor suppressor 1 (LATS1) protein (grey value): 2.33±0.25 vs. 0.98±0.03, phosphorylated Yes-associated protein (p-YAP)/YAP protein ratio (grey value): 2.30±0.35 vs. 1.01±0.05, 14-3-3 protein (grey value):2.19±0.40 vs. 0.99±0.04, all P < 0.05]; Hippo signaling was inhibited by 100 nmol/L of XMU-MP-1 [compared with the blank control group, LATS1 protein (grey value): 0.69±0.10 vs. 0.98±0.03, p-YAP/YAP protein ratio (grey value):0.65±0.06 vs. 1.01±0.05, 14-3-3 protein (grey value): 0.75±0.11 vs. 0.99±0.04, all P < 0.05]. Death of mMSCs was induced by H2O2in a concentration-dependent manner and the minimal effective concentration was 0.1 mmol/L [compared with the blank control group, survival rate of mMSCs: (81.25±11.85)% vs. (100.44±12.39)%, P < 0.05]. Inhibition of Hippo signaling was induced by H2O2in a concentration-dependent manner and the minimal effective concentration was also 0.1 mmol/L [compared with the blank control group, LATS1 protein (grey value): 0.75±0.06 vs. 1.01±0.09, p-YAP/YAP protein ratio (grey value): 0.69±0.05 vs. 0.98±0.05, both P < 0.05], those effects might associate with reduction of Bcl-2/Bax ratio (grey value: 0.48±0.18 vs. 1.06±0.09, P < 0.05). Compared with the treatment of 0.1 mmol/L of H2O2, activation of Hippo signaling by 5 mmol/L of 2-DG [ LATS1 protein (grey value):0.95±0.05 vs. 0.64±0.06, p-YAP/YAP protein ratio (grey value): 0.87±0.03 vs. 0.45±0.16, both P < 0.05] improved survival of mMSCs [(92.80±9.43)% vs. (75.47±9.43)%, P < 0.05] through an increase of Bcl-2/Bax ratio (grey value:1.14±0.16 vs. 0.77±0.12, P < 0.05); however, inhibition of Hippo signaling by 100 nmol/L of XMU-MP-1 [ LATS1 protein (grey value): 0.39±0.03 vs. 0.64±0.06, p-YAP/YAP protein ratio (grey value): 0.28±0.04 vs. 0.45±0.16, both P < 0.05] decreased survival of mMSCs [(57.54±4.59)% vs. (75.47±9.43)%, P < 0.05] through an decrease of Bcl-2/Bax ratio (grey value: 0.63±0.20 vs. 0.77±0.12, P < 0.05). Compared with normal lung tissue, acute respiratory distress syndrome (ARDS) lung tissue markedly activate Hippo signaling in mMSCs [LATS1 protein (grey value): 1.71± 0.08 vs. 1.00±0.10, p-YAP/YAP protein ratio (grey value): 2.46±0.39 vs. 1.01±0.04, 14-3-3 protein (grey value):2.27±0.52 vs. 1.01±0.08, all P < 0.05]. Conclusion Hippo signaling could affect survival and capability of anti-oxidative stress of mMSCs via modulation of Bcl-2/Bax ratio in vitro.

Anti-oxidative stress effects of curcumin on rat models of traumatic brain injury / 中国比较医学杂志

Objective To investigate the changes of content or activity of Nrf2 and anti-oxidative stress-related factors in rat models of traumatic brain injury,and explore the mechanisms of protective effect of curcumin on brain damage and oxidative stress in rats. Methods Twenty healthy SPF male SD rats were divided into 4 groups: the control group, brain injury model group(TBI group),brain injury and solvent-treated group(TBI+S group),brain injury and curcumin-treated group(TBI+C group),5 rats ineach group. The control group received only saline and anesthesia. The TBI,TBI+S and TBI+C groups were given free falling body brain injury modeling device to establish the models and then received curcumin(5 mg/kg), an equal amount of DMSO solvent(0.05%)and an equal amount of physiological saline, respectively. The rats were sacrificed at the next day and the RNA and proteins of brain tissues were extracted. qRT-PCR and Western blot were used to detect the mRNA and protein expression of Nrf2. Chemocolorimetry was used to detect the content or activity of MDA, GSH, CAT and SOD in the brain tissues of rats. ELISA was used to detect the contents of iNOS and HO-1. Results Compared with the control group,the mRNA and protein expressions of Nrf2, the content of MDA,the activity of HO-1 and iNOS were significantly increased,the content of GSH, the activity of SOD and CAT were significantly decreased in the TBI group and TBI+S group,with a significant difference(P< 0.05). Compared with the TBI and TBI+S groups,the mRNA and protein expressions of Nrf2,the content of MDA,the activities of HO-1 and iNOS were significantly decreased,the content of GSH,the activity of SOD and CAT were significantly increased in the TBI+C group,showing a sigfnificant difference(P< 0.05),but there were no significant differences between the TBI group and TBI+S group(P< 0.05). Conclusions Curcumin has an anti-oxidative stress effect on rats with brain injury. It can reduce the expression of Nrf2,change the anti-oxidant stress-related indicators,therefore to protect the TBI-impaired brain tissues.

Effects of p-coumaric acid on preventing acute hypoxia-induced pulmonary edema mice / 中国药学杂志

OBJECTIVE: To study the preventing effects of p-coumaric acid(p-CA) on acute hypoxia-induced pulmonary edema by mice experiments. METHODS: Acute-hypoxia model was established using a normobaric hypoxia chamber in vivo. Salidroside was set as a positive control drug. And the test period was 7 d using a method of intragastric administration. The measurements including pulmonary water content, HE staining, inflammatory factors, anti-oxidative indexes and Na+, K+-ATPase were performed to determine the efficacies and mechanisms of p-CA on preventive against acute hypoxia-induced pulmonary edema. RESULTS: As compared with the normal group, pulmonary water contents increased significantly by 3.56% in the mice treated with acute hypoxia (9.5% O2) for 6 h (control group) (P<0.01), and administration with p-CA (25, 100 mg•kg-1•d-1) for 7 d could significantly reduce this index (P<0.05), which was as effective as the positive group. The action mechanisms of p-CA could be due to its abilities of improving the activity of Na+, K+-ATPase, enhancing antioxidant capacity (SOD↑, CAT↑ and MDA↓) and inhibiting inflammatory factors (IL-1β and IL-6). CONCLUSION: p-CA has greater preventive effects on acute hypoxia-induced pulmonary edema in mice.

Phenolic constituents of Ledum palustre and their anti-oxidant activity / 中草药

To study the chemical constituents of Ledum palustre. Methods Compounds were isolated from n-hexane fraction in the ethanol extract from L. palustre by silica gel column chromatography and HPLC. The structures of the chemical constituents were identified by analysis on their spectral data. Results Fourteen compounds were isolated from EtOAc fraction in the ethanol extract from L. palustre and identified as resveratorol (1), moracin M (2), catechin (3), epicatechin (4), scopoletin (5), esculetin (6), fraxetin (7), 5-hydroxy-2methoxybenzoic acid (8), fraxidin (9), 2α,3α-epoxy-5,7,3’,4’-tetrahydroxyflavan-(4β-8)-epicatechin (10), 2α,3α-epoxy-5,7,3’,4’-tetrahydroxyflavan-(4β-8-catechin) (11), cleomiscosin A (12), cleomiscosin C (13), and isofraxoside (14). Conclusion Compounds 1, 2, and 8-13 are obtained from the plants of Ledum L. for the first time. Compounds 3, 4, 6, 7, 10, and 11 had significant anti-oxidative activities.

Research progress on clinical applications and mechanisms of Guizhi Fuling Prescription in treating cerebrovascular diseases / 中草药

Cerebrovascular diseases (CVD) with high morbidity, disability, mortality, and recurrence rate have become the focus in the medical research. Modern researches have indicated that Guizhi Fuling Prescription (GFP) possesses anti-inflammatory, immune-modulation and anti-oxidative effects and so on. Investigations showed that GFP had been used in the researches of ischemic and hemorrhagic cerebrovascular diseases, mainly used in the clinical research of ischemic cerebrovascular, in which the research of treating acute cerebral infarction gets the best clinical effect of all. In order to provide theoretical reference for intensive research of GFP in treatment of CVD, this article reviewed the research progress in clinical application and mechanisms of GFP in treatment of CVD.

Chemical analysis, antimicrobial and anti-oxidative properties of Daucus gracilis essential oil and its mechanism of action

Objective: To evaluate the essential oils (EO) composition, antimicrobial and antioxi-dant power of a local plant, Daucus gracilis (D. gracilis). Methods: The aerial parts of D. gracilis were subjected to hydro distillation by a Cle-venger apparatus type to obtain the EO which had been analyzed by gas chromatography and gas chromatography coupled with mass spectrometry, and screened for antimicrobial activity against five bacteria and three fungi by agar diffusion method. The mechanism of action of the EO was determined on the susceptible strains by both of time kill assay and lysis experience. The minimal inhibitory concentrations were determined by agar macro-dilution and micro-dilution methods. Anti-oxidative properties of the EO were also studied by free diphenyl-2-picrylhydrazyl radical scavenging and reducing power techniques. Results: The EO yielded 0.68 (v/w). The chemical analysis presented two dominant constituents which were the elemicin (35.3%) and the geranyl acetate (26.8%). D. gracilis EO inhibited the growth of Bacillus cereus and Proteus mirabilis significantly with minimal inhibitory concentrations of 17.15 mg/mL by the agar dilution method and 57.05 mg/mL and 114.1 mg/mL, respectively by liquid micro-dilution. A remarkable decrease in a survival rate as well as in the absorbance in 260 nm was recorded, which suggested that the cytoplasm membrane was one of the targets of the EO. The EO showed, also, important anti-oxidative effects with an IC50 of 0.002 mg/mL and a dose-dependent reducing power. Conclusions: D. gracilis EO showed potent antimicrobial and anti-oxidative activities and had acted on the cytoplasm membrane. These activities could be exploited in the food industry for food preservation.

Chemical analysis, antimicrobial and anti-oxidative properties of Daucus gracilis essential oil and its mechanism of action

Objective: To evaluate the essential oils (EO) composition, antimicrobial and antioxidant power of a local plant, Daucus gracilis (D. gracilis). Methods: The aerial parts of D. gracilis were subjected to hydro distillation by a Clevenger apparatus type to obtain the EO which had been analyzed by gas chromatography and gas chromatography coupled with mass spectrometry, and screened for antimicrobial activity against five bacteria and three fungi by agar diffusion method. The mechanism of action of the EO was determined on the susceptible strains by both of time kill assay and lysis experience. The minimal inhibitory concentrations were determined by agar macro-dilution and micro-dilution methods. Anti-oxidative properties of the EO were also studied by free diphenyl-2-picrylhydrazyl radical scavenging and reducing power techniques. Results: The EO yielded 0.68 (v/w). The chemical analysis presented two dominant constituents which were the elemicin (35.3%) and the geranyl acetate (26.8%). D. gracilis EO inhibited the growth of Bacillus cereus and Proteus mirabilis significantly with minimal inhibitory concentrations of 17.15 μg/mL by the agar dilution method and 57.05 μg/mL and 114.1 μg/mL, respectively by liquid micro-dilution. A remarkable decrease in a survival rate as well as in the absorbance in 260 nm was recorded, which suggested that the cytoplasm membrane was one of the targets of the EO. The EO showed, also, important anti-oxidative effects with an IC

Effects of stocking density and exchanging water frequency on growth and inner quality of Whitmania pigra / 中国中药杂志

The effects of stocking density and exchanging water frequency on growth, digestive enzyme activity, anti-oxidative enzyme and inner quality of Whitmania pigra Whitman were evaluated with corresponding measures. The results showed that the eventual biomass, specific growth rate, gained weight rate, activities of amylase, lipase, protease, SOD, CAT, and ALP correlated positively with stocking density and negatively with exchanging water frequency (P<0.05). Exchanging water frequency had negative correlation with ammonia nitrogen, nitrite, and hydrogen sulfide while revealed positive correlation with dissolved oxygen in the water. Stocking density and exchanging water frequency showed no significant effects on the contents of moisture, total ash, and acid-insoluble ash. It suggested that the optimum stocking density was 7.5 million per hectare and the appropriate exchanging water interval was 72 h.

Investigation of Protective Effect of Phenethyl Alcohol Glycosides Extracted from Herba Cistanchis on Human Sperm DNA with Oxidative Damage by Raman Spectroscopy / 广州中医药大学学报

Objective To study the protective effect of phenethyl alcohol glycosides extracted from Herba Cistan-chis on human sperm DNA with oxidative damage by Raman spectroscopy. Methods The human sperm model of oxidative damage was induced with Fenton’s reagent in vitro. After co-cultured with the phenethyl alcohol gly-cosides extracted from Herba Cistanchis ( in the dosage of 0.1, 0.01 and 0.001 μg/mL) , the changes of the sperm nuclear DNA were observed by using confocal micro-Raman spectroscopy. Results The intensity and peaks of the Raman spectra of the human sperm nuclei treated by Fenton’s reagent were changed significantly, and then the changes of intensity and peaks were inhibited after treatment with phenethyl alcohol glycosides of Herba Cistanchis, the inhibition being dose-dependent. Conclusion The phenylethyl alcohol glycosides ex-tracted from Herba Cistanchis have protective effect on human sperm DNA with oxidative damage.